Chemical Biology Supergroup: Bo Xia, Hubert Huang
Wednesday, October 30, 2024 3:30pm to 4:30pm
About this Event
Speaker: Bo Xia, Xia Lab
Talk Title: High-Throughput In Silico Genetic Screen for Discovering Novel Genome Regulations
Description: High-throughput genetic screening has been instrumental in identifying candidate regulators for mechanistic investigations but frequently faces challenges with feasibility, resource, and time consumption. In this presentation, I will talk about how our development of AI-powered in silico genetic screen approaches can overcome these challenges and accelerate gene regulation discoveries in both normal and disease-related contexts.
Speaker: Hubert Huang, Sellers Lab
Talk Title: E3-substrate tagging by ubiquitin biotinylation
Description: E3 ubiquitin ligases are important therapeutic targets due to their genetic associations with diseases and their roles in targeted protein degradation; however, their physiological functions are largely elusive because the substrates they regulate are often unknown. To address this gap, we developed E-STUB, a novel substrate identification method that integrates proximity labeling with ubiquitin enrichment to enhance substrate detection. Through E-STUB, we gained deep insights into the molecular actions of E3 ligase modulators, such as PROTACs and molecular glues, and successfully identified endogenous substrates of E3 ligases, demonstrating the potential for systematic mapping of E3 ligase-substrate relationships.